Progesterone Saliva ELISA

Intended use

Enzyme immunoassay for the quantitative determination of progesterone in human saliva. For research use only. Not for use in clinical diagnostic procedures.

Test principle

Solid phase enzyme-linked immunosorbent assay (ELISA) based on the competition principle. An unknown amount of antigen present in the sample and a fixed amount of enzyme labelled antigen compete for the binding sites of the antibodies coated onto the wells. After incubation the wells are washed to stop the competition reaction. After the substrate reaction the intensity of the developed colour is inversely proportional to the amount of the antigen in the sample. Results of samples can be determined directly using the standard curve.

The new IBL International Progesterone Saliva ELISA requires as low as 50 µL Saliva sample volume and has a broad standard range up to 5,000 pg/mL.

Advantages of Standardized reagents

Our Saliva ELISA product line has been revised so that the main steroids (testosterone, progesterone, DHEA) may be processed in a similar way:

• The substrate, washing and stop solutions are interchangeable (provided the same lot number for each component is used);
• The enzyme conjugate, the substrate and stop solutions are uniformly pipetted with 100 µL;
• The kits contain as few as 6 standards and 2 controls;
• Only 50 µL sample volume is required;
• All kits use the same sample diluent buffer.

Thanks to the similar processing and assembling, the assays can easily be performed in combination.

Pulsatile dynamics

Due to the pulsatile dynamics of steroid secretion repeated saliva sampling is recommended. IBL recommends collecting 3 to 5 saliva samples within 2 hours. In the laboratory, equal volumes of the individual saliva samples can be mixed. This mixed sample results in a mean free Progesterone value, which represents the active hormone concentration in a reproducible way.

For concrete data please consult the Instruction for Use in the download box on the top right side.