Dehydroepiandrosterone (DHEA) Saliva ELISA
- Regulatory Status
- RUO
- Kit size
- 12 x 8
- Method
- ELISA
- Incubation time
- 1 x 2 h, 1 x 30 min
- Standard range
- 20 - 2160 pg/ml
- Specimen / Volumes
- 50 µL saliva
- Substrate / isotope
- TMB 450 nm
Enzyme immunoassay for the quantitative determination of Dehydroepiandrosterone (DHEA) in human saliva. For research use only. Not for use in diagnostic procedures.
The IBL International DHEA ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with a polyclonal antibody directed towards an antigenic site on the DHEA molecule. Endogenous DHEA of a patient sample competes with a DHEA-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of DHEA in the sample. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of DHEA in the patient sample.
For concrete data please consult the Instruction for Use in the download box on the top right side.
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Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
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