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Intended Use

This LC-MS/MS kit is intended for the determination of Vitamin B1 & B6 in whole blood. The components in this kit must be used as stated in the user manual.

Summary

Mass spectrometry-based methods have been tested for the determination of Vitamin B1 and B6 using a Tandem Mass Spectrometry System (LC-MS/MS). Furthermore, chromatographic separation for both vitamins is critical and not easy. This method can be used for the routine analysis of Vitamin B1 and B6 in whole blood. Sample preparation is simple and rapid and analogous for the different biological matrices.

A six-point lyophilized whole blood calibrator as well as lyophilized blood controls are also available for quality assurance. Both components are added to the “Starter Kit” (30261535) or can be ordered separately. Two isotope-labelled internal standards, Pyridoxal-5’-phosphate (methyl D3) and Thiamin Diphosphate (methyl D3), are added to compensate for matrix effects and measurement variations. Samples are analyzed using positive ion electrospray in MRM mode for maximum sensitivity and selectivity.

Kit Composition

Two different concentrated solvent kits, as well as single components, are available to order.

Starter Kit

This Kit includes everything necessary for a first installation of the Vitamin B1 and B6 LC-MS/MS kit on an LC-MS/MS system. Includes all solvents for HPLC and sample extraction, Calibrators, Controls, as well as one HPLC column and consumables.

Solvent Kit

The Solvent Kit includes a set of solvents to perform the sample extraction and the HPLC. Calibrators, Controls, Consumables, or an HPLC-Column are not included.
Furthermore, all components can be ordered separately.*

Kit Components Table

Our Product Families

Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.

And as experts in laboratory automation, we can support our customers with the protocols for open ELISA platforms.

All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use. 

All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).

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This is who we are.

As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.

Our range of high-quality immunoassays is supported by a diverse portfolio of automated solutions, making us the perfect partner for you and your customers.

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