Fecal Helicobacter pylori-Antigen ELISA

This microplate-based ELISA (enzyme linked immunosorbent assay) kit is intended for the quantitative and qualitative detection of Helicobacter pylori antigen in feces. For research use only. Not for use in clinical diagnostic procedures.

This “sandwich” ELISA is designed, developed and produced for the quantitative measurement of H. pylori antigen in stool specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified anti-H. Pylori antibody onto the wall of microtiter wells. Assay calibrators and fecal specimen are added to microtiter wells of microplate that was coated with a highly purified monoclonal H. pylori antibody on its surface. During the assay, the H. pylori antigen will be bound to the antibody coated plate after an incubation period. The unbound material is washed away and another HRP-conjugated monoclonal antibody, which specifically recognizes the protein of H. pylori is added for further immunoreactions. After an incubation period, the immunocomplex of “H. pylori Antibody – H. pylori Antigen – HRP-conjugated Anti-H. pylori Tracer Antibody” is formed, if H. pylori antigen is present in the test sample. The unbound tracer antibody and other proteins in buffer matrix are removed in the subsequent washing step. HRP conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to H. pylori proteins captured on the wall of each microtiter well is directly proportional to the amount of H. pylori antigen level in each test specimen.