ACTH ELISA
- Regulatory Status
- RUO
- Kit size
- 12 x 8
- Method
- ELISA
- Incubation time
- 1 x 2 h, 1 x 20 min
- Standard range
- 11 - 416 pg/mL
- Specimen / Volumes
- 200 µL EDTA-Plasma
- Substrate / isotope
- TMB 450 nm
Enzyme immunoassay is used in the quantitative determination of human adrenocorticotropic hormone (ACTH) in EDTA plasma. For research use only. Not for use in clinical diagnostic procedures. This ELISA kit is designed, developed, and produced for the quantitative measurement of human ACTH in an EDTA plasma sample. The assay utilizes the two-site “sandwich” technique with selected antibodies that bind to N-terminal and C-terminal epitopes of ACTH. Assay standards, controls and patient samples are added directly to wells of a microtiter plate that is coated with antibodies to the Cterminal of human ACTH. Immediately, a horseradish peroxidase (HRP) conjugated anti-N-terminal of human ACTH antibody is added to each well. After the first incubation period, a “sandwich” of solidphase polyclonal antibody - human ACTH – HRP conjugated monoclonal antibody” is formed. The unbound antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction, which is terminated with an acidic reagent (i.e. ELISA stop solution). The absorbance is then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human ACTH in the test sample. A standard curve is generated by plotting the absorbance versus the respective human ACTH concentration for each standard on a point-to-point or 4-parameter curve fitting. The concentration of human ACTH in test samples is determined directly from this standard curve.
IBL presents a new, simplified adrenocorticotropic hormone (ACTH) ELISA, with the following
advantages:
- Assay time of 2 hours
- Easy calculation of results (single plate reading at 450 nm)
- Wide Standard range (0 - 416 ng/mL) Synonyms: Adrenocorticotropic hormone, Corticotropin, Cosyntropin
For concrete data please consult the Instruction for Use in the download box on the top right side.
For concrete data please consult the Instruction for Use in the download box on the top right side.
Our Product Families
Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
And as experts in laboratory automation, we can support our customers with the protocols for open ELISA platforms.
All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).
This is who we are.
As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
Our range of high-quality immunoassays is supported by a diverse portfolio of automated solutions, making us the perfect partner for you and your customers.
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