25-OH-Vitamin D ELISA
- Regulatory Status
- RUO
- Kit size
- 12 x 8
- Method
- ELISA
- Incubation time
- 1 x 90min., 1 x 30min., 1 x 15min.
- Standard range
- 0 - 130 ng/mL
- Specimen / Volumes
- 25µL serum
- Substrate / isotope
- TMB 450nm
Vitamin D refers to a group of fat-soluble secosteroids being responsible for intestinal absorption of calcium, iron, magnesium, phosphate and zinc. The most important vitamin Ds in humans are vitamin D3 (cholecalciferol) and vitamin D2 (ergocalciferol). Vitamin D3 is synthesized in the skin from the cholesterol precursor 7-dehydrocholesterol and is the major source of vitamin D in humans. In contrast, vitamin D2 is mainly a synthetic product, which is predominantly present as a food additive in certain foods such as milk. In the liver vitamin D is further metabolized via hydroxylation to 25-hydroxyvitamin D (25(OH)-vitamin D). 25(OH)-vitamin D is the major circulating metabolite of vitamin D. 25(OH)-vitamin D is the precursor for other vitamin D metabolites, but shows also limited activity by itself. The most active metabolite is 1,25-dihydroxyvitamin D, which is produced in the kidney by 1-hydroxylation of 25(OH) vitamin D.
Advantages of our assay are:
- Direct assay without extraction
- Ready-to-use reagents
- Easily adaptable on open ELISA automates
For concrete data please consult the Instruction for Use in the download box on the top right side.
- Kennel, K. A.; Drake, M. T.; Hurley, D. L. Vitamin D Deficiency in Adults: When to Test and How to Treat. Mayo Clin. Proc. 2010, 85 (8), 752–757; quiz 757–758.
- Holick, M. F.; Binkley, N. C.; Bischoff-Ferrari, H. A.; Gordon, C. M.; Hanley, D. A.; Heaney, R. P.; Murad, M. H.; Weaver, C. M. Evaluation, Treatment, and Prevention of Vitamin D Deficiency: An Endocrine Society Clinical Practice Guideline. J. Clin. Endocrinol. Metab. 2011, 96 (7), 1911–1930.
- Wranicz, J.; Szostak-Węgierek, D. Health Outcomes of Vitamin D. Part II. Role in Prevention of Diseases. Rocz. Panstw. Zakl. Hig. 2014, 65(4), 273-279.
- Christakos, S.; Ajibade, D. V.; Dhawan, P.; Fechner, A. J.; Mady, L. J. Vitamin D: Metabolism. Endocrinol. Metab. Clin. North Am. 2010, 39 (2), 243–253.
- Martineau, A. R.; Forouhi, N. G. Vitamin D for COVID-19: A Case to Answer? Lancet Diabetes Endocrinol. 2020, 8 (9), 735–736. https://doi.org/10.1016/S2213-8587(20)30268-0
Our Product Families
Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
And as experts in laboratory automation, we can support our customers with the protocols for open ELISA platforms.
All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).
This is who we are.
As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
Our range of high-quality immunoassays is supported by a diverse portfolio of automated solutions, making us the perfect partner for you and your customers.
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At Tecan, we are driven to improve people’s lives and health.