Cortisol ELISA
- Regulatory Status
- RUO
- Kit size
- 12 x 8
- Method
- ELISA
- Incubation time
- 1 x 1 h, 1 x 15 min
- Standard range
- 20 - 800 ng/mL
- Specimen / Volumes
- 20 µL serum, plasma
- Substrate / isotope
- TMB 450 nm
Enzyme immunoassay for the quantitative determination of Cortisol in human serum and plasma. For research use only. Not for use in clinical diagnostic procedures.
Solid phase enzyme-linked immunosorbent assay (ELISA) based on the competition principle. An unknown amount of antigen present in the sample and a fixed amount of enzyme labelled antigen compete for the binding sites of the antibodies coated onto the wells. After incubation the wells are washed to stop the competition reaction. After the substrate reaction the intensity of the developed colour is inversely proportional to the amount of the antigen in the sample. Results of samples can be determined directly using the standard curve.
Cortisol (hydrocortisone, compound F) is the main glucocorticoid in humans and is produced in the zona fasciculata of the adrenal cortex. 90 % of the circulating cortisol are bound to corticoid binding globulin (CBG, Transcortin), ca. 7 % are bound to albumin and only 1–3 % are unbound. Only the latter part represents the active form of cortisol. In humans there is a physiological fluctuation of cortisol achieving the highest level in the morning and the lowest during midnight. This fluctuation of cortisol plasma level is reflected in saliva normally with a peak in the first 90 minutes after wake up.
For concrete data please consult the Instruction for Use in the download box on the top right side.
(1) Immunoassay Handbook, 4.; Wild, D., Ed.; Elsevier: Amsterdam, 2013.
(2) Thomas, L. Labor Und Diagnose, 8.; TH-Books-Verl.-Ges: Frankfurt, 2012.
(3) Clow, A.; Thorn, L.; Evans, P.; Hucklebridge, F. The Awakening Cortisol Response: Methodological Issues and Significance. Stress 2004, 7 (1), 29–37. https://doi.org/10.1080/10253890410001667205.
(4) Nieman, L. K.; Biller, B. M. K.; Findling, J. W.; Newell-Price, J.; Savage, M. O.; Stewart, P. M.; Montori, V. M. The Diagnosis of Cushing’s Syndrome: An Endocrine Society Clinical Practice Guideline. J. Clin. Endocrinol. Metab. 2008, 93
(5), 1526–1540. https://doi.org/10.1210/jc.2008-0125. (5) Bornstein, S. R.; Allolio, B.; Arlt, W.; Barthel, A.; Don-Wauchope, A.; Hammer, G. D.; Husebye, E. S.; Merke, D. P.; Murad, M. H.; Stratakis, C. A.; Torpy, D. J. Diagnosis and Treatment of Primary Adrenal Insufficiency: An Endocrine Society Clinical Practice Guideline. J. Clin. Endocrinol. Metab. 2016, 101 (2), 364–389. https://doi.org/10.1210/jc.2015-1710.
Our Product Families
Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
And as experts in laboratory automation, we can support our customers with the protocols for open ELISA platforms.
All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).
This is who we are.
As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
Our range of high-quality immunoassays is supported by a diverse portfolio of automated solutions, making us the perfect partner for you and your customers.
Happy to help.
At Tecan, we are driven to improve people’s lives and health.