Acetylcholine Receptor-Ab (ARAb) Blocking RIA
- Regulatory Status
- RUO
- Kit size
- 100
- Method
- RIA
- Incubation time
- 1 x 3h, 1 x 1 h
- Standard range
- negative and positive control
- Specimen / Volumes
- 20 µL serum
- Substrate / isotope
- 125I < 150 kBq
Radio immunoassay for the quantitative determination of blocking autoantibodies against the acetylcholine receptor in human serum. For research use only. Not for use in clinical diagnostic procedure.
The samples are incubated with labeled 125I–alpha-bungarotoxin acetylcholine receptors. The blocking acetylcholine receptor antibodies contained in the samples compete with the 125I–alpha-bungarotoxin for a limited number of binding sites on the receptors. If samples are positive, blocking antibodies bind to the acetylcholine receptors. The resulting complexes of acetylcholine receptors and specific antibodies are precipitated with Con-A Sepharose. After centrifugation and aspiration of the supernatant the precipitates are counted for 125I by use of a gamma counter. The measured counts are inversely proportional to the concentration of blocking acetylcholine receptor autoantibodies present in the sample. The concentration is expressed as the activity of inhibition (%) of the binding of 125I-alpha-bungarotoxin to the receptors.
For concrete data please consult the Instruction for Use in the download box on the top right side.
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Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
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All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
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