| instructions for use | certificate | MSDS |
|---|---|---|
Radio immunoassay for the quantitative determination of cAMP in human plasma and urine. The test can also be used for applications in other biological materials. For research use only. Not for use in clinical diagnostic procedures.
This assay is based on the principle of radioimmunoassays for cyclic nucleotides described by Steiner et al. (1972), modified by Harper and Brooker (1975). The kit includes two standard ranges. The less sensitive method (1.5-450 pmol/mL) is applicable for urine and other samples with high cAMP levels. The highly sensitive method (0.05-15 pmol/mL) includes a simple acetylation step and is suitable for plasma and tissue extracts.
In the cAMP radioimmunoassay the samples (or standards) are incubated over-night with 125I-labeled cAMP and the pre-precipitated antiserum. There is competition between the radioactive and the non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125I-labeled antigen bound to the antibody is inversely proportional to the analysed concentration of the sample. When the system is in equilibrium, the reaction is stopped by the addition of a co-precipitating solution and a centrifugation step. The precipitate is counted in a gamma counter. Quantification of unknowns is achieved by comparing their activity with a response curve prepared using known standards. Because of its high specificity and sensitivity the assay is also suitable for the determination of cAMP in the adenylate cyclase assay in the presence of high concentrations of ATP.
Cyclic adenosin-3',5'-monophosphate (cAMP) serves as a second messenger in signal transduction of various hormones, like adrenaline, ACTH, LH, FSH, glucagon and calcitonin. The hormone itself is the first messenger, binding to specific membrane receptors of hormone-responsive cells. This leads to the activation of an enzyme located at the inner side of the cell membrane, called adenylate cyclase, which catalyses the synthesis of cAMP from ATP. The increased cellular level of cAMP initiates the activation of protein kinases.
For concrete data please consult the Instruction for Use in the download box on the top right side.
Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
And as experts in laboratory automation, we can support our customers with the protocols for open ELISA platforms.
All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).
As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
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