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Interleukin-5 / IL-5 (mouse) ELISA

Catalog no.BE45051
Regulatory Status
RUO
Kit size
12 x 8
Method
ELISA
Incubation time
1 x 2 h, 1 x 1 h, 1 x 10 min
Standard range
7.8 - 500 pg/mL
Specimen / Volumes
50 µL serum, cell culture supernatant et al.
Substrate / isotope
TMB 450 nm

Mouse IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stew cells, induction of mIL-2R expression on both normal splenic and activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. mIL-5 has been shown to be produced exclusively by the TH2 subset of mouse TH clones. The spectrum of biological activities of mIL-5, on B cells as well as other cell types suggests that mIL-5 plays an important functional role in the activities of TH2 cells. mIL-5 is a heavily glycosylated glycoprotein. Synonyms: B cell differentiation factor 1, TRF, EDF

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