Serotonin RUO
- Regulatory Status
- RUO
- Kit size
- 12 x 8
- Method
- ELISA
- Incubation time
- 1 x 20 min, 1 x 30 min, 1 x 15 min, 1 x 15± min
- Standard range
- 30 – 1500 ng/ml
- Specimen / Volumes
- 10 µl serum, 10 µL urine, 20 µl plasma
- Substrate / isotope
- TMB 450 nm
Enzyme Immunoassay for the quantitative Determination of Serotonin in human Serum, EDTA-Plasma and Urine. For research use only. Not for use in diagnostic procedures.
Serotonin (5-Hydroxytryptamine), a biogenic amine, is a product of the tryptophan metabolism. It is a well-evaluated neurotransmitter of the central nervous system and can be found in high concentrations in the chromaffin cells of the intestinal mucosa, in the platelets and in the serotonergic neurons of the brain. Central-serotonergic neurons influence physiological functions such as sleep and the hormonal and cardio-vascular regulation.
After sample preparation in the preparation plate, the derivatization takes place in the ELISA plate. In this process, serotonin is quantitatively converted into N-acyl serotonin by the acylating reagent.
The Serotonin ELISA is a competitive enzyme immunoassay. Antigens bound to the solid phase and free antigens in solution compete for a defined number of antibody binding sites. When the system is in equilibrium, the unbound antigen-antibody complex is removed in a washing step and the correspondingly bound complex is detected using a peroxidase conjugate and determined via the conversion of tetramethylbenzidine (TMB).
Our Product Families
Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
And as experts in laboratory automation, we can support our customers with the protocols for open ELISA platforms.
All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).
This is who we are.
As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
Our range of high-quality immunoassays is supported by a diverse portfolio of automated solutions, making us the perfect partner for you and your customers.
Happy to help.
At Tecan, we are driven to improve people’s lives and health.