BetaPrion® ELISA
- Regulatory Status
- RUO
- Kit size
- 12 x 8
- Method
- ELISA
- Incubation time
- 2 x 1h, 1 x 15min
- Standard range
- 1 - 20 ng/mL
- Specimen / Volumes
- 10µL CSF, other cell free biological samples
- Substrate / isotope
- TMB 450nm
| instructions for use |
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Test principle
The BetaPrion® HUMAN ELISA is based on a sensitive sandwich ELISA using two specific monoclonal anti-human prion protein antibodies. The capture antibody, which is immobilized on the surface area of the microtiter plate, specifically recognizes the conformational epitope of human prion protein. Prion protein from human CSF, serum or plasma samples, standards and controls bound by the capture antibody is detected by a HRP-conjugated mAb that specifically binds to amino acids 151-180 (RYYRENMHRY) of human prion protein (Dorey, et al., 2015). Amount of bound conjugated antibody is estimated using chromogenic substrate tetramethylbenzidine (TMB). The concentration of prion protein is proportional to the obtained optical density. Controls are included for the proof of reproducibility and evaluation of the assay within labs.
Distributed by TECAN, IBL International
For concrete data please consult the Instruction for Use in the download box on the top right side.
Our Product Families
Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
And as experts in laboratory automation, we can support our customers with the protocols for open ELISA platforms.
All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).
This is who we are.
As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
Our range of high-quality immunoassays is supported by a diverse portfolio of automated solutions, making us the perfect partner for you and your customers.
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