T4 Total ELISA
- Regulatory Status
- IVD
- Kit size
- 12 x 8
- Method
- ELISA
- Incubation time
- 1 x 5 / 1 x 80 / 1 x 10 min
- Standard range
- 0 nmol/L – 250 nmol/L
- Specimen / Volumes
- 10 µl Serum / Plasma
- Substrate / isotope
- TMB 450 nm
| instructions for use |
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Intended Use
The DRG T4 Total ELISA is an enzyme immunoassay for the quantitative in vitro diagnostic measurement of total Thyroxin (T4) in serum or plasma (EDTA, lithium heparin or citrate plasma).
Summary and Explanation
The thyroid hormones thyroxine (T4) and triiodothyronine (T3), are synthesized and stored in the thyroid gland after stimulation by thyroid-stimulating hormone (TSH) from the anterior pituitary gland and thyrotropin-releasing hormone (TRH) from the hypothalamus. Proteolytic cleavage of follicular thyroglobulin releases T4 into the bloodstream. The major form of thyroid hormone in the blood is T4, which has a longer half-life than T3. In humans, the ratio of T4 to T3 released into the blood is approximately 14:1. T4 is converted to the active T3 (three to four times more potent than T4) within cells by deiodinases. More than 99% of T4 is reversibly bound to the plasma proteins thyroxine binding globulin (TBG; 70%) thyroxine binding pre-albumin (TBPA; 20%), and albumin (10%).
T3 and T3 exert powerful and essential regulatory influences on growth, differentiation, cellular metabolism, and general hormonal balance of the body.
T4 is the basal parameter to evaluate hormone secretion of the thyroid gland. Diseases affecting thyroid function may present a wide array of confusing symptoms. Measurement of total T4, TSH, free T3 and free T4 by immunoassay are reliable and convenient methods to determine the presence of thyroid disorders in patients. Increased levels of T4 have been found in hyperthyroidism due to Grave's disease and Plummer's disease and in acute and subacute thyroiditis. Low levels of T4 have been associated with congenital hypothyroidism, myxedema, chronic thryoiditis (Hashimoto's disease), and with some genetic abnormalities.
NOT INTENDED FOR NEWBORN SCREENING.
For concrete data please consult the Instruction for Use in the download box on the top right side.
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Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
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All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).
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As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
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