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The human IFN-omega ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IFN-omega in cell supernatant, serum and plasma (EDTA,citrate and heparin). For research use only. Not for use in diagnostic procedures.
An anti-human IFN-omega coating antibody is adsorbed onto microwells. Human IFN-omega present in the sample or standard binds to antibodies adsorbed to the microwells. A biotin-conjugated anti-human IFN-omega antibody is added and binds to human IFN-omega captured by the first antibody. Following incubation unbound biotinconjugated anti-human IFN-omega antibody is removed during a wash step. Streptavidin- HRP is added and binds to the biotinconjugated anti-human IFN-omega antibody. Following incubation unbound Streptavidin- HRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human IFN-omega present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 human IFN-omega standard dilutions and human IFN-omega sample concentration determined.
Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
And as experts in laboratory automation, we can support our customers with the protocols for open ELISA platforms.
All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).
As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
Our range of high-quality immunoassays is supported by a diverse portfolio of automated solutions, making us the perfect partner for you and your customers.
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