Enzyme immunoassay for the quantitative measurement of 17beta-Estradiol in human saliva.
17β-Estradiol (Estradiol; E2) is the most important natural estrogen. It is present in females and males. In the former, the estrogens stimulate the growth of sex organs and the development of secondary sexual characteristics, and they also affect the gonadotropin secretion. 17β-Estradiol measurements are useful to detect estrogen deficiency in delayed puberty, primary and secondary amenorrhea and in postmenopausal women mostly.
The new IBL International 17ß-Estradiol Saliva ELISA is calibrated to the LC-MS/MS reference method and includes harmonized reagents within the IBL Saliva ELISA product line.
Benefits of the 17ß-Estradiol Saliva ELISA
Calibration to the LC-MS/MS;
Only 50 µL Sample volume needed;
Same sample diluent within the salivary steroid ELISA line;
Defined reference values: monthly profiles for menstruating women and normal values for postmenopausal women and men
ELISA vs Mass Spectronomy
Mass spectrometry (MS) is considered as the reference for steroid hormone quantification. This methodology does however represents a large investment and as such is not available to all laboratories. The main IBL saliva assays have been developed to correlate to this reference method, and even more importantly, only require low sample volume for quantification of the steroid hormones.Figure: Excellent correlation of IBL International 17ß-Estradiol Saliva ELISA to the LC-MS/MS reference method
Consistent and reliable processing within Saliva product line
Our Saliva ELISA product line (Testosterone, Progesterone, 17ß-Estradiol, Estriol, DHEA) can be processed in a similar way and be easily performed in combination:
The TMB, washing and stop solutions are interchangeable;*
The kits contain as few as 6 standards and 2 controls;
Only 50 µL (max 100 µL) sample volume are required;
All kits use the same sample diluent buffer.
*provided the same lot number for each component is used
Physiology and Time Dependent Concentration
Estradiol is secreted into circulation by the ovaries, placenta, adrenal gland, and testes or is produced by extra-glandular conversion of secreted androgen precursors. Estradiol is biologically the most active of the naturally produced human estrogens. In the postmenopausal women, Estradiol originates from extra-glandular conversion of androgens and circulates in low, non-cyclic concentrations.The changes in the hormone levels are registered during the different the phases of the menstrual cycle.
Figure: Reference values measured with the IBL 17ß-Estradiol saliva ELISA. Saliva samples were collected from 28 women (using no contraceptive). They collected five samples a day during a period of 2 hours after awakening covering the whole menstrual cycle with a maximum of 30 days. Samples were pooled per day and the estradiol concentration was measured to obtain a daily value throughout the menstrual cycle.
Similar to Testosterone and Progesterone, 17ß-Estradiol also shows short time pulsating dynamics, especially in females. Therefore single saliva determinations will result in arbitrary values. We recommend collecting 3 to 5 saliva samples within 2 hours. In the laboratory, equal volumes of the individual saliva samples can be mixed. This mixed sample results in a mean 17ß-Estradiol value, which represents the active hormone concentration in a reproducible way.
Applications of 17ß-Estradiol determination
In women, the estrogens stimulate the growth of sex organs and the development of secondary sexual characteristics, and they also affect the gonadotropin secretion. In the males, the role of 17β-Estradiol is less well defined although it seems to be involved in the regulation of gonadotropin secretion. In non-pregnant women 17β-Estradiol is almost exclusively produced by ovary. Especially after having changed into the menopause, estrogens are produced by the liver, the brain, the muscles and by the adipose tissue, too. Moreover, the measurement of 17β-Estradiol is helpful to determine a lack of estrogens, which may be expressed as delayed puberty, primary and secondary amenorrhoea, and occurs in the menopause. Other hormone assays are required for proper interpretation and differential diagnosis. The concentration of gonadotropin should be measured to find the origin of the lack of estrogens (synthesis or regulation). The determination of 17β-Estradiol is useful for the diagnosis of pubertas praecox of girls. Concerning men, the level of 17β-Estradiol may be used for the differential diagnosis of gynecomastia.
Measurement of 17ß-Estradiol in saliva
Being principally bound to sex hormone binding globulin (SHBG) and to serum albumin, only 1 - 3 % of estradiol circulating in plasma is present in its free form. Only this portion represents the active part in the endocrine regulation. The free hormone is released in equal amounts in saliva.
Therefore measuring the 17ß-Estradiol in saliva is a convenient non-invasive method. Easier sample collection without repeated venipunctures and reduced stress on the specimen offer benefits to the patient while sensibility and accuracy of the results are ensured.
For concrete data please consult the Instruction for Use in the download box on the top right side.
References:
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