Metanephrine ELISA
- Regulatory Status
- RUO
- Kit size
- 12 x 8
- Method
- ELISA
- Incubation time
- 1 x 1h, 1 x 30 min, 1 x 40 min
- Standard range
- 0 - 2500 µg/L
- Specimen / Volumes
- 10 µL urine
- Substrate / isotope
- PNPP 405 nm
Enzyme immunoassay for the quantitative determination of metanephrine in human urine. The test serves as an aid of diagnosis of catecholamine-secreting pheochromocytomas and paragangliomas as well as monitoring tool for therapy follow-up. Patient population includes adults suspected to be affected by neuroendocrine tumors. Pre-test in case of genetic predisposition (e.g. Von-Hippel-disease, and multiple endocrine neoplasia type 2) is advised.
The test kit is intended for professional use only. The test kit is not for self-testing. The Metanephrine ELISA is NOT intended for near patient testing. The test kit is intended for manual use and can be adapted to open ELISA processors.
The assay procedure follows the basic principle of competitive ELISA whereby there is competition between a biotinylated and a non-biotinylated antigen for a fixed number of antibody binding sites. The amount of biotinylated antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the free biotinylated antigen is removed by a washing step and the antibody bound biotinylated antigen is determined by use of streptavidin alkaline phosphatase as marker and p-nitrophenyl phosphate as substrate. Quantification of unknowns is achieved by comparing the enzymatic activity of unknowns with a response curve prepared by using known standards.
For concrete data please consult the Instruction for Use in the download box on the top right side.
Our Product Families
Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
And as experts in laboratory automation, we can support our customers with the protocols for open ELISA platforms.
All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).
This is who we are.
As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
Our range of high-quality immunoassays is supported by a diverse portfolio of automated solutions, making us the perfect partner for you and your customers.
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At Tecan, we are driven to improve people’s lives and health.