Methylmalonic Acid Concentrated LC-MS Kit - Starter Kit
- Regulatory Status
- RUO
- Kit size
- 100
- Method
- LC-MS
- Incubation time
- LC-MS run time: 6 minutes
- Specimen / Volumes
- 100 µl Serum / Plasma (EDTA or Heparin)
- Substrate / isotope
- N/A
Intended Use
The LC-MS/MS kit is intended for the determination of Methylmalonic Acid in serum or plasma. The components in the kit must be used as stated in the user manual.
Summary
Mass spectrometry-based methods have been widely tested for the determination of Methylmalonic Acid. Furthermore, chromatographic separation from the structural isomer, Succinic Acid (SA), is critical and not elementary. This method can be used for the analysis of Methylmalonic Acid in serum and plasma. Sample preparation is simple and rapid. An isotope-labelled internal standard (Methylmalonic Acid D3) is added to compensate for matrix effects and measurement variations.
Methylmalonic Acid is determined from human serum or plasma by UHPLC with negative ion electrospray LC-MS/MS. Prior to the LC-MS/MS analysis, a sample clean-up is performed to remove the sample matrix and to spike with the internal standard. After separation by chromatography on an analytical C-18 column, Methylmalonic acid is ionized by electrospray ionization (ESI) and detected by LC-MS/MS. Measurement of the analytes is carried out in Multi Reaction Monitoring (MRM) mode. In this mode, only selected ions (known as precursor ions) with a defined mass/charge (m/z) ratio are isolated in the first quadrupole and subsequently transferred into the collision cell, where they are fragmented by impact with an inert gas (argon or nitrogen) at defined voltage settings. Among the fragments generated (known as product ions), only those with a defined m/z ratio can pass the third quadrupole for final detection. The analytical method enables a quantitation in biological matrices by use of isotope isotope-labelled internal standard.
Kit Composition
Two different concentrated solvent kits, as well as single components, are available to order.
Starter Kit
This Kit includes everything necessary for a first installation of the Methylmalonic LC-MS/MS kit on an LC-MS/MS system. Includes all solvents for HPLC and sample extraction, Calibrators, Controls, as well as one HPLC column and consumables.
Solvent Kit
The Solvent Kit includes a set of solvents to perform the sample extraction and the HPLC. Calibrators, Controls, Consumables, or an HPLC-Column are not included.
Furthermore, all components can be ordered separately.*
Kit Components Table
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Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
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