Human Titin N-Fragment (Serum) ELISA

Titin: the giant protein.

It is one of sarcomere structured protein that is a minimum unit of myofibrillary protein and it has a role as an elastic protein for recovering the length of shortened sarcomere by its contraction. It has been known that titin is cleaved by protein metabolic enzymes such as calpain and matrix metalloprotease if muscles are damaged.

Actually, massive titin degradation was observed in the skeletal muscle of patients with muscular dystrophy, and the presence of various titin fragments in the blood and urine was confirmed. In particular, it has been reported that the approximately 26 kDa N-fragment on the N-terminal region and the approximately 12 kDa C-fragment on the C-terminal region found in urine are increased in patients with various muscle diseases including muscular dystrophy.

This kit is a sandwich ELISA with a combination of two antibodies against titin N-fragments. A titin fragment containing N-terminal region in human serum can be measured.

Titin is the largest protein (3800kDa) in the human body and is found in muscle cells. It acts like a spring, helping muscles stretch and then return to their normal shape. Titin is the biggest known protein in the human body and plays a key role in making muscles strong and flexible.

Titin in the context of longevity.

Loss of muscle mass and function (sarcopenia) is one of the major drivers of frailty and loss of independence during aging. Titin N-Fragment is an emerging biomarker of muscle degradation that enables early assessment and monitoring of muscle health.

Sarcopenia is defined as follows:

• Age-related disease involving progressive loss of skeletal muscle mass, strength and physical function.
• Diagnosable and treatable condition, with muscle mass index as a key measurement tool.
• It affects approximately 16–29% of individuals over 80 years of age.

Titin N-Fragment can be measured in urine or serum as a biomarker of interest in research related to muscle protein turnover and skeletal muscle biology.

Sarcopenia, frailty and muscle decline are active areas of scientific research, with studies investigating factors such as nutrition and physical activity.

Urine and serum-based assays provide laboratories with tools for the quantitative measurement of Titin N-Fragment. The Titin N-Fragment ELISAs are used for the analysis of muscle-related biomarkers in urine and serum samples.

Sarcopenia and frailty are not inevitable. Evidence highlights the preventive impact of a protein-rich diet, vitamin D and targeted exercise[1][2].

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