Vitamin B1 & B6 LC-MS Kit

Intended purpose
This LC-MS/MS kit is intended for the determination of Vitamin B1 & B6 in whole blood. The components in this kit must be used as stated in the user manual.

Summary
Water-soluble B Vitamins are important cofactors in cell metabolism. Two water-soluble vitamins with clinical relevance are Vitamins B1 and B6. Thiamine Diphosphate (TDP) is the biologically active form of Vitamin B1 and is required for various metabolic functions. Prolonged deficiency can cause Beriberi, a debilitating neurological disease.1 Pyridoxal 5-phosphate (PLP) is the biologically active form of Vitamin B6 and is a coenzyme for a number of transamination reactions. It plays critical roles in chronic disease and pro-inflammatory response.2 Additionally, both Vitamin B6 and B1 have also been linked to increased survival rates in the elderly.

Thiamine deficiency may give rise to manifestations in the cardiovascular and nervous systems. There are two forms; dry Beriberi, which consists of sensorimotor neuropathy and Wernicke-Korsakoff syndrome, and wet Beriberi, which consists of edema and cognitive heart failure, but little CNS manifestations. The clinical picture of Wernicke’s encephalopathy, with or without Korsakoff syndrome, is frequently encountered in alcoholics, with predominant oculomotor and cerebellar symptomatology, although it can also be seen in other conditions, such as hyperemesis, dialyses, and post-gastrointestinal surgery.

Pyridoxine deficiency on a nutritional basis has been recognized as a rare cause of severe and even fatal convulsions in neonates and infants. Pyridoxine dependency develops during fetal life as a genetic disorder and causes both intrauterine and postnatal seizures.

Neurologic disorders reflecting both pyridoxine deficiency and pyridoxine toxicity have been recognized. Both overdose and deficiency may cause peripheral neuropathy. Pyridoxine deficiency causes injury of motor and sensory axons, whereas an overdose of pyridoxine causes a pure sensory neuropathy or neuronopathy with sensory ataxia.

Mass spectrometry based methods have been tested for the determination of Vitamin B1 & B6. Furthermore chromatographic separation for both of the vitamins is critical and not easy.

This method can be used for the routine analysis of Vitamin B1 & B6 in whole blood. Sample preparation is simple and rapid and analogous for the different biological matrices. A six-point lyophilized whole blood calibrator at clinically relevant levels has been added to the kit. Lyophilized blood controls are also available for quality assurance.

Two isotope-labelled internal standard Pyridoxal-5’-phosphate (methyl D3) and Vitamin B1 pyrophosphate (methyl D3) are added to compensate for matrix effects and measurement variations. Samples are analyzed using positive ion electrospray in MRM mode for maximum sensitivity and selectivity

Manufactured by Diagnotix; Distributed by Tecan, IBL International GmbH.

For concrete data please consult the Instruction for Use in the download box on the top right side.

Für konkrete Daten konsultieren Sie bitte die Arbeitsanweisung in der Download Box oben auf der rechten Seite.