Insulin total (mouse/rat) ELISA

Original article #27705 Insulin is a peptide hormone that is released from the beta cells of the islets of Langerhans in the pancreas. It is synthesized as a 86 amino-acid precursor “proinsulin” and it is cleaved into C-peptide and insulin that consists of a 21 amino-acid alpha-chain and a 30 amino-acid beta-chain that are linked by disulfide binding due to activation of prohormone conversion enzyme (PC2, PC3) in tehsecretory granule. Insulin deposits glucose and promotes the synthesis of proteins in skeletal muscles. It also has physiological function such as suppressing gluconeogenesis in liver and it has been known as the only hormone that can decrease blood glucose. Insulin is constantly released to maintain the level of blood glucose, while it is also released in large amounts upon incretin stimulation and high glucose levels to suppress quickly increased blood glucose after a meal. However, lack of insulin secretion and decreasing insulin sensitivity are observed and eventually high blood glucose is observed in diabetic conditions. Measuring insulin in fasting (F-IRI) is required for clinical assessment of diabetes such as function of insulin secretion and insulin resistance. This ELISA kit can measure mouse and rat total insulin including proinsulin and low concentration of insulin in fasting using tiny amount of samples. Separate control set available on request