1,25-Dihydroxy-Vitamine D ELISA*
- Regulatory Status
- EU: CE
- Kit size
- 12 x 8
- Method
- ELISA
- Incubation time
- 1 x 24 h
- Standard range
- 5 - 544 pmol/L
- Specimen / Volumes
- 500 µL serum, plasma/extraction
- Substrate / isotope
- TMB 450 nm
*This assay is only available in restricted areas. Please inquire.
Vitamin D3 is mainly synthesized in the skin from 7- dehydrocholesterol and is partially from dietary origin. By hydroxylation in the liver the intermediate product 25-OH Vitamin D3 is produced. The intermediate product must then be metabolized further before it can carry out the functions of Vitamin D on intestine, kidney and bone. This subsequent reaction takes exclusively place in the kidney in nonpregnant mammals. It is further hydroxylated to produce 1,25 dihydroxy-Vitamin D3 (1,25-(OH)2D3).
In addition to renal tissue, placenta of pregnant women and macrophage cells in case of sarcoidis can also produce some amount of 1,25-(OH)2D3.
Due to its functions in the bone and intestine, it must be considered as a hormone. This hormone stimulates the intestinal absorption of both calcium and phosphorus. It also stimulates bone resorption and mineralization, thereby preventing the development of rickets and osteomalacia.
For concrete data please consult the Instruction for Use in the download box on the top right side.
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Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
And as experts in laboratory automation, we can support our customers with the protocols for open ELISA platforms, such as the Freedom EVOlyzer or Thunderbolt®.
All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).
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As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
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