PEth LC-MS Kit
- Regulatory Status
- RUO
- Kit size
- 200
- Method
- LC-MS
- Incubation time
- LC-MS run time: 5.0 min
- Specimen / Volumes
- 100 µl Whole blood
- Substrate / isotope
- NA
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| instructions for use |
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Intended Use
This for Research Use Only product is intended to assist in the detection of previous ethanol exposure by the quantitative determination of different phosphatidylethanol (PEth) species in whole blood, using LC-MS. The product is not intended for self-testing, companion diagnostic use, or as a fully automated test.Background
Phosphatidylethanols (PEths) are abnormal phospholipids formed only in the presence of ethanol, via phospholipase D-mediated transphosphatidylation of phosphatidylcholine in red blood cell membranes [1,2]. Among over 40 known PEth homologues, POPEth (PEth 16:0/18:1) and PLPEth (PEth 16:0/18:2) are the most abundant and analytically robust [3]. They can be detected in whole blood for up to approximately 2–4 weeks after the last alcohol intake, with concentrations correlating strongly with the amount and frequency of consumption [4–5]. Quantification by LC–ESI–MS/MS offers high analytical specificity and sensitivity, with sub-ng/mL detection limits.LC-MS Method
PLPEth and POPEth are determined from human whole blood by UHPLC with negative ion electrospray LC-MS/MS. Prior to the LC-MS/MS analysis, a sample clean-up is performed to remove the sample matrix and to spike with the internal standard.Manufactured by Diagnotix; Distributed by Tecan, IBL International GmbH. For concrete data please consult the Instruction for Use in the download box on the top right side.
For concrete data please consult the Instruction for Use in the download box on the top right side.
- Cabarcos P, et al. Determination of direct alcohol markers: a review. Anal Bioanal Chem. 2015;407:4907–4925.
- Varga A, et al. Phosphatidylethanol in blood as a marker of ethanol consumption. Alcohol Alcohol. 2014;49(2):147–154.
- Helander A, Zheng Y. Molecular species of the alcohol biomarker phosphatidylethanol in human blood measured by LC-MS. Clin Chem. 2009;55(7):1395–1405.
- Kechagias S, et al. PEth in blood as a marker of alcohol consumption: comparison with CDT, GGT and MCV. Alcohol Alcohol. 2015;50(5):509–513.
- Walther L, et al. Phosphatidylethanol in blood as a marker of chronic alcohol use: a systematic review. Addiction. 2015;110(9):1473–1483.
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