PLGF ELISA
- Regulatory Status
- EU: CE
- Kit size
- 12 x 8
- Method
- ELISA
- Incubation time
- 1 x 1 h, 3 x 30 min
- Standard range
- 25 - 1000 pg/mL
- Specimen / Volumes
- 25 µL serum
- Substrate / isotope
- TMB 450 nm
Intended Use
Enzyme immunoassay for the quantitative determination of human placenta growth factor (PLGF) in human serum.
Principle of the Test
The PLGF ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle.
The microtiter wells are coated with a monoclonal antibody directed towards a unique antigenic site of the PLGF molecule. An aliquot of patient sample containing endogenous PLGF is incubated in the coated well.
After a washing step a biotin-linked polyclonal antibody specific for PLGF is added to the wells. Following a wash to remove any unbound antibody a streptavidin HRP enzyme complex is added to the wells. After incubation the unbound enzyme complex is washed off. The amount of bound peroxidase is proportional to the concentration of PLGF in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of PLGF in the patient sample.
Summary and Explanation
Angiogenesis and vascular transformation are important processes in the normal development of the placenta. Abnormal angiogenesis and vascular transformation are considered to be one of the main reasons for preeclamptic pregnancies and intrauterine growth retardation. Placental growth factor PLGF, a member of the VEGF family, is produced mainly by the placenta and is a potent angiogenic factor. The corresponding receptor, the soluble soluble fms-like tyrosine kinase-1 is considered to have angiogenic properties.
For concrete data please consult the Instruction for Use in the download box on the top right side.
Our Product Families
Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
And as experts in laboratory automation, we can support our customers with the protocols for open ELISA platforms, such as the Freedom EVOlyzer or Thunderbolt®.
All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).
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As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
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