Free Testosterone RIA (CT)
- Regulatory Status
- EU: CE
- Kit size
- 96 Tests
- Method
- RIA (CT)
- Incubation time
- 1 x 2 h (37°C)
- Standard range
- 0.3 - 90 pg/mL
- Specimen / Volumes
- 50 µL Serum
- Substrate / isotope
- 125I < 185 kBq
| instructions for use |
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Radioimmunoassay (CT) for the quantitative determination of Free Testosterone (FT) in human serum.
Free testosterone diffuses through cell membranes and binds to specific receptor proteins (androgen receptors); the Testosterone-receptor complexes act as transcriptional modulators on cis-regulatory regions of many genes.
Excess of Androgens in women causes hirsutism and signs of virilization; Testosterone level in serum has to be determined before and after ovarian and adrenal stimulation and supression to identify the source of excessive hormone production.
Primary and secondary hypogonadism in men result in clinical hypoandrogenization, correlated with the degree of gonadal failure in Testosterone production. The determination of serum Testosterone together with that of LH allows the correct assessement of those conditions.
The diagnosis of true anorchia also requires to discriminate this condition from cryptorchidism. Under prolonged hCG stimulation, Testosterone levels remain very low in true anorchia while cryptorchid testes can respond to stimulation.
Androgen resistance syndromes, due to X linked androgen receptor gene deficiencies, are made of various degrees of sexual ambiguity. Whatever the severity of the phenotypical abnormalities, serum Testosterone is systematically high in regards to elevated LH serum levels in these conditions.
Testosterone assays include total testosterone (direct, extraction, coated tubes) and free testosterone determinations.
Total Testosterone in plasma includes free Testosterone and Testosterone bound to SHBG, albumin, CBG. The mean percentage of each in normal men is 2.7, 32, 65 and <0.1 respectively.
Solvents break the protein binding in extraction assays whereas blocking agents release Testosterone from proteins in direct assays. The advantage of a free testosterone assay is that free testosterone concentrations are in equilibrium with testosterone bound to receptors in the organs.
For concrete data please consult the Instruction for Use in the download box on the top right side.
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Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
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All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
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As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
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