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ApoB-100 ELISA

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Catalog no.JP27181
Regulatory Status
RUO
Kit size
12 x 8
Method
ELISA
Incubation time
1 x 1h, 2 x 30min.
Standard range
0.13 - 8.4 µg/mL
Specimen / Volumes
100 µL serum, EDTA-plasma, cell culture supernatant
Substrate / isotope
TMB 450nm

Although lipids are not soluble in water, apolipoproteins are carrier proteins binding to lipids forming lipoprotein particles, that are water-soluble and can be carried through water-based circulation (i.e., blood, lymph). Apolipoproteins are classified according to their forms and functions, and there are six major classes and several sub-classes. ApoB (ApolipoproteinB) is the primary apolipoprotein of Low-Density-Lipoproteins (LDL) and is considered to reflect changes in LDL levels in blood. There are two types of apolipoproteins B. The first is ApoB-100, which correlates with cholesterol levels and is mainly released as part of LDL and VLDL. The second is ApoB-48, which is part of chylomicrons and correlates with tryglyceride levels. In general, concentration of ApoB in blood is determined as sum of ApoB-100 and ApoB-48. This kit measures solely ApoB-100 in plasma samples by using ApoB-100 specific antibodies, that don't cross react to ApoB-48. This highly sensitive sandwich ELISA can also measure ApoB-100 in cell culture media or various fractionated lipoprotein samples. This product is for research use only and not for clinical diagnostic procedures.

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Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.

And as experts in laboratory automation, we can support our customers with the protocols for open ELISA platforms, such as the Freedom EVOlyzer or Thunderbolt®.

All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use. 

All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).

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As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.

Our range of high-quality immunoassays is supported by a diverse portfolio of automated solutions, making us the perfect partner for you and your customers.

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