PECAM-1 ELISA
- Regulatory Status
- RUO
- Kit size
- 12 x 8
- Method
- ELISA
- Incubation time
- 1 x 3 h, 1 x 10 min
- Standard range
- 0.47 - 30 ng/mL
- Specimen / Volumes
- 10 µL serum, plasma, urine, cell culture supernatant et al.
- Substrate / isotope
- TMB 450 nm
PECAM-1 (platelet endothelial cell adhesion molecule-1)
is a newly characterized adhesion molecule that belongs to the immunoglobulin superfamily. PECAM-1 is a transmembrane glycoprotein with a molecular weight of approximately 130 kDa, depending on the degree of glycosylation. PECAM-1 is constitutively expressed on all vascular cells. It has also been found on platelets, monocytes, neutrophils, CD8+ T cells, bone marrow stem cells, and transformed cell lines of the myeloid and megakaryocytic lineage. Interestingly, PECAM-1 was also detected on human, mouse, and rat solid tumor lines [16]. Recent studies suggest a role for PECAM-1 in the inflammatory process and leukocyte-endothelial interaction. PECAM-1 appears to be able to interact both with itself (homophilic binding) and with other "non-PECAM-1" molecules (heterophilic binding). PECAM-1 is an early and sensitive marker for tumor-induced angiogenesis. Besides the membrane-bound form of PECAM-1 a soluble form of the molecule exists, which is 5-10 kDa smaller than cell-associated PECAM-1, and contains the cytoplasmic tail. Soluble PECAM-1 was detected in normal human plasma.
Applications
- Antibodies against PECAM-1 have been shown to exhibit a high degree of sensitivity and specificity for endothelial cells in normal, inflammatory and neoplastic tissue.
- PECAM-1 is a sensitive and specific marker for detection of melanoma-associated angiogenesis. Thus, PECAM-1 may be a useful marker for early detection of tumor metastasis.
- Compared to the normal gut, there is a significant increase of PECAM-1-positive vessels in the mucosa of uninvolved ulcerative colitis [15].
- In gingivitis and periodontitis lesion the expression of PECAM-1 on mononuclear infiltrates increases significantly with increasing size of infiltrate.
Synonyms: CD31, EndoCAM
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As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
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