Intended purpose
This LC-MS/MS kit is intended for the determination of Vitamin A & E in Serum / Plasma.
The components in this kit must be used as stated in the user manual.
Summary
Vitamins A and E are fat-soluble vitamins, which perform important functions in the body.
Vitamin A plays an important role in vision, bone metabolism, and the synthesis of steroid hormones. Deficiency of vitamin A can lead to night blindness, skin dehydration, and hair loss.
Vitamin E protects, among others, unsaturated fatty acids in the cell membranes and the LDL cholesterol from attacks by reactive radicals, which can occur as a result of increased oxidative stress in the body. Deficiency of vitamin E can lead to permanent joint pain, making the determination of vitamin E levels essential in this case.
However, overdoses of these vitamins may cause hypervitaminosis with intoxication symptoms. In addition, chemotherapies should not be "accompanied" by extra vitamin supplements – especially high doses of vitamins A and E may limit the success of treatment.
Mass spectrometry based methods have been tested for the determination of Vitamin A & E. Furthermore chromatographic separation for both of the vitamins is critical and not easy. This method can be used for the routine analysis of Vitamin A & E in serum / plasma. Sample preparation is simple and rapid and analogous for the different biological matrices. A six-point lyophilized serum calibrator at clinically relevant levels has been added to the kit. Lyophilized serum controls are also available for quality assurance.
Two isotope-labelled internal standards, Vitamin A-D6 and Vitamin E-D3, are added to compensate for matrix effects and measurement variations. Samples are analyzed using positive ion electrospray in (Multiple Reaction Monitoring) MRM mode for maximum sensitivity and selectivity.
Two isotope-labelled internal standards, Vitamin A-D6 and Vitamin E-D3, are added to compensate for matrix effects and measurement variations. Samples are analyzed using positive ion electrospray in (Multiple Reaction Monitoring) MRM mode for maximum sensitivity and selectivity.
Manufactured by Diagnotix; Distributed by Tecan, IBL International GmbH.
For concrete data please consult the Instruction for Use in the download box on the top right side.
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