Transglutaminase (tTG) IgA ELISA
- Regulatory Status
- EU: CE
- Kit size
- 12 x 8
- Method
- ELISA
- Incubation time
- 1 x 30 min, 2 x 15 min
- Standard range
- 0 - 300 U/mL, cut-off 15 U/mL
- Specimen / Volumes
- 10 µL serum
- Substrate / isotope
- TMB 450 nm
The present enzyme-linked immuno sorbent assay (ELISA)
is intended for the quantitative or qualitative determination of IgA antibodies in human serum or plasma (EDTA, citrate, heparin), directed against tTG (tissue Transglutaminase).
The immobilised antigen is a highly purified preparation of human recombinant tTG, expressed in the baculovirus system. The test is fast (incubation time 30 / 30 / 30 minutes) and flexible (divisible solid phase, ready to use reagents). Six calibrators allow quantitative measurements; a negative and a positive control check the assay performance.>/p>
Celiac disease (CD; synonyme: gluten-sensitive enteropathy) affects the upper small intestine and is caused by a hypersensitive reaction to gluten, a set of proteins present in many kinds of cereal grain, e.g. wheat, oats, barley and rye. Its morphological manifestation, the more or less complete atrophy of the villi of the mucous membrane, leads to malabsorption problems, e.g. chronic vitamin deficiency. It has been known for many years that elevated levels of gluten-specific antibodies occur in the sera of celiac patients. Also, autoantibodies directed against endomysium of the smooth muscle are specifically associated with this disease. Tissue transglutaminase (tTG) has been identified as the predominant endomysial CD autoantigen.
For concrete data please consult the Instruction for Use in the download box on the top right side.
Our Product Families
Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
And as experts in laboratory automation, we can support our customers with the protocols for open ELISA platforms, such as the Freedom EVOlyzer or Thunderbolt®.
All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).
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As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
Our range of high-quality immunoassays is supported by a diverse portfolio of automated solutions, making us the perfect partner for you and your customers.
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