Estriol High Sensitive Saliva ELISA

The Estriol Sensitive Saliva ELISA will be IVDR ready soon!

Saliva Testing of hormone levels

Enzyme immunoassay for the quantitative determination of Estriol in human saliva.

The Estriol high sensitive Saliva ELISA is intended for the quantitative measurement of Estriol in human saliva.

Estriol is an estrogen typically produced during pregnancy. Estriol level found in non-pregnant women are similar to level both during pre- and post-menopause and are similar to the level in men. The estriol saliva assay is commonly applied to monitor estriol level in women who take supplements as part of a hormone replacement therapy and as an indicator of premature delivery in pregnant women. However, this assay is not intended to be used in the context of screening for congenital disorders of the fetus (such as Down Syndrome).

Additonal hormone assays are recommended for an interpretation of the level of estriol.

The Estriol high sensitive Saliva ELISA is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding and measured on an absorbance reader. The assay is semi-automated requiring general purpose laboratory instruments and consumables such as absorbance microplate reader/washer, vortexer and pipettes to execute the test. The assay is adaptable by laboratory personnel to automate on open ELISA based liquid handler platforms; however, the programming of the steps and timing required must be verified by the laboratory. Test results are calculated from a standard curve and compared to laboratory established reference ranges from healthy adults (i.e. normal ranges).

The test kit is intended for professional laboratory use by trained personnel. The test kit is not for self-testing. The Estriol high sensitive Saliva ELISA is not intended for near-patient testing.

Benefits of the Estriol high sensitive Saliva ELISA

• Calibration to the LC-MS/MS;
• Only 100 µL Sample volume needed;
• Results available within 3 hours;
• Sensitive assay for measurement of males and females;
• Defined reference values.

ELISA vs. Mass Spectrometry

Mass spectrometry (MS) is considered as the reference for steroid hormone quantification. This methodology does however represents a large investment and as such, is not available to all laboratories. The main IBL saliva assays have been developed to correlate to this reference method, and even more importantly, only require low sample volume for quantification of the steroid hormones.

Consistent and reliable processing within Saliva product line

Our Saliva ELISA product line (Cortisol, Testosterone, Progesterone, 17ß-Estradiol, Estriol, DHEA) can be processed in a similar way and be easily performed in combination:

• The TMB, stop solution and wash solution have the same design within Cortisol, Testosterone, DHEA, Progesterone and 17ß-Estradiol saliva ELISA (RE52611, RE5263, RE52651 and RE52281 and 30121045)*
• The kits contain as few as 6 standards and 2 controls;
• Only 100 µL sample volume are required; 
• All kits use the same sample diluent buffer.

*Valid lot number of TMB, stop solution and wash solution are stated on the outer label and on the Quality Control certificates.

Automation 

The IBL International Estriol high sensitive Saliva ELISA (Cat. No. 30121046) can be adapted on different ELISA processors. Open automated platforms like the EVOlyzer® from Tecan have been tested and show good correlation to the manual runs:

For concrete data please consult the Instruction for Use in the download box on the top right side.