IgG Food Screen 24 ELISA
- Regulatory Status
- RUO
- Kit size
- 15x3x32
- Method
- ELISA
- Incubation time
- 1x1h; 1x30min; 1x15min
- Standard range
- 0.35 - 100 U/mL
- Specimen / Volumes
- Human serum and plasma (EDTA, Citrate, Heparin); 40 μL
- Substrate / isotope
- 450 nm
Enzyme immunoassay for the simultaneous, quantitative measurement of food specific IgG antibodies against 24 allergens in human serum and plasma (EDTA, Citrate, Heparin).
For research use only. Not for use in diagnostic procedures.
TEST PRINCIPLE
The IgG Food Screen 24 ELISA is a conventional ELISA system (Enzyme Linked Immunosorbent Assay). Onto the surface of each cavity of a microtiter plate antigens from foodstuff or foodstuff mixes are immobilized. For the test, a serum or plasma sample is diluted and transferred to the microtiter well. Specific IgG (IgG) antibodies will bind to the corresponding antigens of the foodstuff extract. Bound human IgG is detected by a specific horseradish peroxidase (HRP) conjugated anti human IgG antibody. After addition of the substrate TMB (3,3‘,5,5‘-Tetramethylbenzidine) bound IgG antibodies are visualized by a blue coloration of the solution. Addition of stop solution interrupts the reaction and the blue color turns into a yellow staining. Its intensity correlates with the amount of bound antibody. The yellow coloration is quantified via photometric measurement.
For concrete data please consult the Instruction for Use in the download box on the top right side.
Our Product Families
Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
And as experts in laboratory automation, we can support our customers with the protocols for open ELISA platforms, such as the Freedom EVOlyzer or Thunderbolt®.
All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).
This is who we are.
As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
Our range of high-quality immunoassays is supported by a diverse portfolio of automated solutions, making us the perfect partner for you and your customers.
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