PEth Concentrated LC-MS Kit - Solvent Kit

Intended Use

This Research Use Only product is intended to assist in the detection of previous ethanol exposure by the quantitative determination of different Phosphatidylethanol (PEth) species in whole blood, using LC-MS. The product is not intended for self-testing, companion diagnostic use, or as a fully automated test.

Summary

PLPEth and POPEth are determined from human whole blood by UHPLC with negative ion electrospray LC-MS/MS.
Prior to the LC-MS/MS analysis, a sample clean-up is performed to remove the sample matrix and to spike with the internal standard. After separation by chromatography on an analytical C-18 column, PLPEth and POPEth are ionized by electrospray ionization (ESI) and detected by LC-MS/MS. Electrospray ionization is a soft ionization technique where a strong electric field is applied to the liquid passing through the ESI-capillary of the MS-source. The ions are mostly formed in solution before desorption and then transferred into the ion path of the tandem mass spectrometer, which consists of three quadrupoles (two mass selectors connected by a collision cell).
Measurement of the analytes is carried out in MRM (Multiple Reaction Monitoring) mode. In this mode, only selected ions (precursor ions) with a defined mass/charge (m/z) ratio are isolated in the first quadrupole and subsequently transferred into the collision cell, where they are fragmented by impact with an inert gas (argon or nitrogen) at defined voltage settings. Among the fragments generated (known as product ions), only those with a defined m/z ratio can pass the third quadrupole for final detection. In this way, the MRM mode ensures a selective identification and quantification of the target analytes.

Measurands

• PLPEth  (1-Palmitoyl-2-linoleoyl-sn-glycero-3-phosphoethanol)
• POPEth  (1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanol)

Kit Composition

Two different concentrated kits, as well as single components, are available to order.

Starter Kit

This Kit includes all solvents for HPLC and sample extraction, calibrators and controls.

Solvent Kit

The Solvent Kit includes all solvents required for sample extraction and liquid chromatography, including the deproteinization solution and mobile phases. Calibrators, controls, consumables, or a chromatographic column are not included.
All listed components can be ordered separately by the order number mentioned below. An HPLC column and a consumables set are also available separately.

Kit Components Table

Distributed by Tecan, IBL International GmbH.
Please consult Tecan or you local Tecan Distributor for further information and availability.