Intended Use
The LC-MS/MS kit is intended for the determination of Cortisol and Cortisone in urine and saliva
Summary
Cortisol and Cortisone are determined from human urine or saliva by UHPLC with positive ion electrospray LC-MS/MS. Prior to the LC-MS/MS analysis a sample clean-up is performed to remove the sample matrix and to spike with the internal standard. After separation by chromatography on an analytical C-18 column, Cortisol and Cortisone are ionized by electrospray ionization (ESI) and detected by LC-MS/MS. Electrospray ionization is a soft ionization technique where a strong electric field is applied to the liquid passing through the ESI-capillary of the MS-source. The ions are mostly formed in solution before desorption and then transferred into the ion path of the tandem mass spectrometer, which consists of three quadrupoles (two mass selectors connected by a collision cell). Measurement of the analytes is carried out in MRM mode. (MRM: Multiple Reaction Monitoring). In this mode, only selected ions (precursor ions) with a defined mass/charge (M/z) ratio are isolated in the first quadrupole and subsequently transferred into the collision cell, where they are fragmented by impact with an inert gas (argon or nitrogen) at defined voltage settings. Among the fragments generated (known as product ions), only those with a defined M/z ratio can pass the third quadrupole for final detection. In this way, the MRM mode ensures a selective identification and qualification of the target analytes.
Kit Composition
Two different concentrated solvent kits, as well as single components, are available to order.
Starter Kit
This Kit includes everything necessary for a first installation of the Cortisol and Cortisone LC-MS/MS kit on an LC-MS/MS system. Includes all solvents for HPLC and sample extraction, Calibrators, Controls, as well as one HPLC column and consumables.
Solvent Kit
The Solvent Kit includes a set of solvents to perform the sample extraction and the HPLC. Calibrators, Controls, Consumables, or an HPLC-Column are not included.
Furthermore, all components can be ordered separately.*
Kit Components Table