Parietalcell-Ab ELISA
- Regulatory Status
- EU: CE
- Kit size
- 12 x 8
- Method
- ELISA
- Incubation time
- 3 x 30 min, 1x5min
- Standard range
- 0 - 300 U/mL, cut-off 15 U/mL
- Specimen / Volumes
- 10 µL serum
- Substrate / isotope
- TMB 450 nm
Parietalcell-Ab ELISA is a solid phase enzyme immunoassay employing purified native H+ /K+ –ATPase for the separate quantitative and qualitative detection of antibodies against parietal cells in human serum. Antibodies against parietal cells can be found in pernicious anemia. Pernicious anemia, the most common cause of vitamin B12 deficiency in Western populations, is characterized by pathological lesions of the fundus and body of the stomach. Gastric mucosal atrophy, selective loss of parietal and chief cells from mucosa and lymphocytic infiltration of the submucosa is the typical picture of this disease. Pernicious anemia is characterized by autoantibodies against the hydrogen transporting enzyme H+ /K+ –ATPase of gastric parietal cells, which is responsible for the acidification of the stomach lumen. Anti-parietal cell autoantibodies can be detected by immunofluorescence in 80-90 % of Patients with pernicious anemia, but can also be found in 2-5% of healthy individuals. ELISA test systems for detection of these autoantibodies show a sensitivity of about 80 % and a specificity of about 90%.
For concrete data please consult the Instruction for Use in the download box on the top right side.
Our Product Families
Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
And as experts in laboratory automation, we can support our customers with the protocols for open ELISA platforms, such as the Freedom EVOlyzer or Thunderbolt®.
All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).
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As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
Our range of high-quality immunoassays is supported by a diverse portfolio of automated solutions, making us the perfect partner for you and your customers.
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