Cholin IgG/IgM ELISA
- Regulatory Status
- EU: CE
- Kit size
- 12x8
- Method
- ELISA
- Incubation time
- 3x30min
- Standard range
- 0-300 U/mL, cutoff 12 U/mL
- Specimen / Volumes
- 10 µL serum
- Substrate / isotope
- TMB 450 nm
Intended Use
Cholin IgG/IgM ELISA is a solid phase enzyme immunoassay with highly purified phosphatidyl-cholin and native human ß2-glycoprotein I for the quantitative and qualitative detection of IgG and/or IgM antibodies against phosphatidyl cholin in human serum. These antibodies recognize specific epitopes on a complex composed out of phosphatidyl-cholin and ß2-glyco-protein I.
The assay is an aid in the diagnosis and risk estimation of thrombosis in patients with systemic lupus erythematosus (SLE) and the antiphospholipid syndrome (APS).
Clinical Application and Principle of the Assay
Antibodies against phosphatidyl-choline belong to the group of anti-phospholipid antibodies specific for phospholipids such as cardiolipin, phosphatidyl- serine, -inositol, sphingomyelin and phosphatidic acid. Phospholipids are components of biological membranes.
Anti-phospholipid antibodies are frequently found in sera of patients with systemic lupus erythematosus (SLE) and related diseases. The occurrence of anti-phospholipid antibodies in patients with SLE and related diseases is typical for a secondary anti-phospholipid syndrome (APS). In contrast, anti-phospholipid antibodies in patients with no other autoimmune diseases characterize the primary APS.
Many studies have shown a correlation between these autoantibodies and an enhanced incidence of thrombosis, thrombocytopenia and habitual abortions (as a consequence of placental infarction). The exact mechanisms by which pathogenic anti-phospholipid antibodies induce thrombosis is not yet revealed fully.
Principle of the test
Serum samples diluted 1:101 are incubated in the microplates coated with the specific antigen. Patient´s antibodies, if present in the specimen, bind to the antigen. The unbound fraction is washed off in the following step. Afterwards anti-human immunoglobulins conjugated to horseradish peroxidase (conjugate) are incubated and react with the antigen-antibody complex of the samples in the microplates. Unbound conjugate is washed off in the following step. Addition of TMB-substrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The intensity of color formation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of the respective antibodies in the patient sample.
For concrete data please consult the Instruction for Use in the download box on the top right side.
- Noori EB. Phospholipid autoantibodies-Phosphatidylserine. In: Peter JB, Shoenfeld Y, eds. Autoantibodies. Elsevier Science BV 1996: 630-634.
- Boey, M.L., Colaco, C.B., Gharavi, A.E., et al. (1983). Thrombosis in systemic lupus erythematosus: striking association with the presence of circulating lupus anticoagulant. Br. Med. J. 287: 1021-1023.
- Gastineau, D.A., Kazmier, F.J., Nichols, W.L., Bowie, E.J. (1985). Lupus anticoagulant: an analysis of the clinical and laboratory features of 219 cases. Am. J. Hematol. 19: 265-267.
- McNeil HP, Simpson RJ, Chesterman CN, Kirilis SA (1990). Anti-phospholipid antibodies are directed against a complex antigen that includes a lipuid-binding inhibitor of coagulation: ß2-Glycoprotein I (apolipoprotein H). Proc Natl Acad Sci USA 87: 4120-4124.
- Wöhrle R, Matthias T, von Landenberg P, Oppermann M, Helmke K, Förger F (2000). Clinical relevance of antibodies against different phospholipids. Journal of Autoimmunity 15: A60
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