Cortisol Saliva ELISA

Cortisol Saliva ELISA is IVDR compliant!

Sensitive and reliable

The IBL International Cortisol Saliva ELISA kit is an excellent alternative to the current complex and relatively expensive LC-MS/MS method to determine cortisol in saliva as accurately as possible.

Advantages of the IBL Cortisol Saliva ELISA

• Calibration to the LC-MS/MS reference method;
     IBL-ELISA = 1.015 x LCMS-0.028, R=0.99
• As few as 6 standards and 2 controls;
• Simple processing;
• Clearly defined reference values.

Diurnal rhythm

The normal cortisol levels in human saliva depend on the time of day. The timing of the cortisol peak, however, does not depend on the actual time of day or daylight, but correlates with the usual wake-up time. The new reference ranges have been carefully determined on the basis of 100 day profiles. Starting with batch no. ECO137, the following reference values have to be considered:

Sensitive and reliable

The investigation of cortisol in saliva is indicated in the diagnostic work-up of clinical issues in various scientific/medical fields, particularly endocrinology, psychology, sports medicine, pediatrics, anti-aging medicine and veterinary medicine

Measuring the free cortisol levels in saliva is a very simple and convenient way to check the function of the adrenal gland. Persistently elevated or decreased cortisol levels are often found in patients with adrenal dysfunction. Adrenal dysfunction can occur in many other diseases, such as fibromyalgia, hypothyroidism, chronic fatigue syndrome and premature menopause.
The salivary cortisol test can also be used in patients with conditions such as reduced stress tolerance, fatigue, sleep disorders and depression.

Standardized reagents

Our Saliva ELISA product line has been revised so that the main steroids (cortisol, testosterone, progesterone, DHEA) may be processed in a similar way:

• The substrate, washing and stop solutions are interchangeable;
• The enzyme conjugate, the substrate and stop solutions are uniformly pipetted with 100 µL;
• The kits contain as few as 6 standards and 2 controls;
• Only 50 µL sample volume are required;
• All kits use the same sample diluent buffer.

Thanks to the similar processing and assembling, the assays can easily be performed in combination.

*Product availability and regulatory status may vary across regions outside the EU depending on local country-specific registration. Consult with your Tecan associate for further information.

For concrete data please consult the Instruction for Use in the download box on the top right side.