Melatonin direct Serum/Plasma/Saliva RIA (Non-Extraction)

Radio immunoassay for the in-vitro diagnostic quantitative determination of melatonin in human serum and plasma. Further the test can be used for research of saliva samples. The pineal gland (corpus pineale) has been called a neuroendocrine transducer because of its important role in photoperiodism. The major hormone of the pineal gland is N-acetyl-5-methoxy-tryptamine or melatonin which is synthezised from the amino acid tryptophane. Melatonin has its highest levels in plasma during nighttime. Its characteristic nocturnal surge appears to encode temporal information such as length of night. Regulation of the melatonin secretion is under neural control. Sympathetic innervation seems to play a major role via its release of noradrenaline. Altered patterns and/or levels of melatonin secretion have been reported to coincide with sleep disorders, jet lag, depression, stress, schizophrenia, hypothalamic amenorrhea, pregnancy, anorexia nervosa, some forms of cancer, immunological disorders as well as control of sexual maturation during puberty. Most of the circulating melatonin is metabolized in the liver to 6-hydroxymelatonin and subsequently to 6 sulfatoxymelatonin which is excreted into the urine. The concentration of 6-hydroxymelatonin sulfate in urine correlates well with the total level of melatonin in the blood during the collection period.