Aldosterone RIA (CT)

Radioimmunoassay for the quantitative determination of Aldosterone in human serum and urine.

for in vitro diagnostic use only

Aldosterone is a steroid hormone with molecular weight of 360 produced by the zona glomerulosa of the adrenal cortex. The aldosterone assay is extremely important in the diagnosis of primary hyperaldosteronism (Conn's disease), in the so-called essential hypertension, in arterial pressure changes induced by the administration of abnormal amounts of certain types of food (liquorice) or drugs (potassium/sodium), in nephrosis syndromes etc. Blood levels of aldosterone follow also a circadian rythm and are strictly related to states of rest or exercise, it is necessary to study blood aldosterone levels by means of stimulation and/or suppression dynamic tests.

The present method is based on a competitive radioimmunoassay (RIA). During the incubation, the sample/calibrator aldosterone competes with the aldosterone labeled with Iodine 125 (tracer) for the specific sites of the antiserum coated on the tubes. Separation is based on the use of antibody coated tubes, where the anti-aldosterone antiserum is fixed on the tube walls. After aspiration, the radioactivity in the tubes is measured in a gamma counter. The degree of binding will be inversely proportional to the sample/calibrator hormone concentration.

For concrete data please consult the Instruction for Use in the download box on the top right side.