GLP-1 (9-36/37) ELISA

GLP-1 (Glucagon Like Peptide-1) is a transcriptional product of proglucagon gene as same as glucagon and one of gastrointestinal hormone which is secreted from L cells of lower section of the small intestine. GLP-1 (7-36) amide and GLP-1 (7-37) are active form and these peptides are dissolved in blood by DDP4 (dipeptidyl peptidase-4) into GLP-1 (9-36) amide and GLP-1 (9-37) and these peptides lose its activities in short period. GLP-1 is known as incretin as same as GIP (Glucose-dependent Insulinotropic Polypeptide), it promotes secretion of insulin from β cells of the isles of Langerhans in the pancreas as glucose dependently. GLP-1 also suppresses secretion of glucagon from α cell of pancreas and concentration of glucose in blood after meal. Drug of type 2 diabetes has been developed by utilizing the functional mechanism of GLP-1 such as blood sugar control through pancreas, delayed gastric emptying and appetite suppression which contributes weight loss as extra-pancreatic effects. This ELISA kit can quantitatively measure inactive forms of GLP-1 such as GLP-1 (9-36) amide and GLP-1 (9-37) specifically. It can also quantitatively measure as total GLP-1 in EDTA-plasma which does not contains DPP4 inhibitor since active forms GLP-1 in blood are rapidly transformed to inactive forms in 2 to 5 minutes in a half period. This ELISA kit can quantitatively measure DPP4-cleaved forms of GLP-1 (GLP-1 (9-36) amide and GLP-1 (9-37)). It can also quantitatively measure total GLP-1 in EDTA-plasma which does not contain a DPP4 inhibitor since active forms GLP-1 in blood are rapidly transformed to inactive forms with a half life of 2 to 5 minutes.