sAPP ß-wild type (mouse) ELISA
- Regulatory Status
- RUO
- Kit size
- 12x8
- Method
- ELISA
- Incubation time
- 1x18h, 1x1h, 1x30min
- Standard range
- 13 - 800 pg/mL
- Specimen / Volumes
- 10 µL mouse plasma, cell culture supernatant
- Substrate / isotope
- TMB 450 nm
Studying beta-secretase
activity in-vivo
In Alzheimer's disease, the knowledge about the proteolysis of amyloid precursor protein (APP) is of utmost importance for the development of therapeutic agents. An important step in amyloid beta production is the cleavage of APP by beta secretase (BACE1). It cleaves off the sAPPbeta from the entire protein. sAPPbeta is present in different isoforms. The most frequent naturally occurring variant of sAPPbeta is the non-mutated wild-type isoform.
sAPPbeta (wild-type) is determined to investigate the activity of BACE1 as well as the efficacy of its inhibition.
BACE1 is a very interesting drug target in the development of therapeutic agents and is already being tested by various pharmaceutical companies1,2).
Up to now it was not possible to determine the endogenous sAPPbeta (wild-type) cleavage product in mice. For the first time now, however, our sAPPbeta (wild-type) mouse ELISA makes it possible to directly trace the beta-cleavage activity in a mouse model.
Characteristics of ELISA:
- Sample volume: 10 µL mouse plasma
- Analytical sensitivity: 2.87 pg/mL
- Measuring range: 13 – 800 pg/mL
References
- 1) Vassar R. BACE1 inhibitor drugs in clinical trials for Alzheimer's disease. Alzheimers Res Ther. 2014;6(9):89.
- 2) Yan R, Vassar R. Targeting the β secretase BACE1 for Alzheimer's disease therapy. Lancet Neurol. 2014 Mar;13(3):319-29. Distributed by IBL International
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Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
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As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
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