Cytochrome-C ELISA

Apoptotic cell death is a fundamental feature of virtually all cells. It is an indispensable process during normal development, tissue homeostasis, development of the nervous system and the regulation of the immune system. Insufficient or excessive cell death can contribute to human disease, including cancer or degenerative disorders. The highly coordinated and stereotyped manner of this induced cell death suggests that the cells activate a common death program, towards which diverse signal – transducing pathways converge. The mitochondria turned out to participate in the central control or executioner phase of the cell death cascade. Cytochrome c was identified as a component required for the crucial steps in apoptosis, caspase-3 activation and DNA fragmentation. Cytochrome c was shown to redistribute from mitochondria to cytosol during apoptosis in intact cells. Measurement of cytochrome c release from the mitochondria is a tool to detect the first early steps for initiating apoptosis in cells. Cytochrome c release in the cytosol occurs prior to the activation of caspases and DNA fragmentation which is considered the hallmark of apoptosis. Detection of cytochrome c released from the mitochondria to the cytoplasm can be achieved by a selective lysis of the cell membrane. Very recently it has been shown that this mitochondria dwelling molecule can be detected in the medium already 1h after apoptosis. Moreover, elevated cytochrome c levels were observed in serum from patients with hematological malignancies. In the course of cancer chemotherapy, the serum-cytochrome c level grew rapidly and it decreased gradually as the patient was cleared from malignant cells. Thus, serum-cytochrome c monitoring might serve as a clinical marker indicating the onset of apoptosis and cell turn-over in vivo.