Immunosuppressants Concentrated LC-MS Kit - Solvent Kit
- Regulatory Status
- RUO
- Kit size
- 300
- Method
- LC-MS
- Incubation time
- LC-MS run time: 4 min
- Specimen / Volumes
- 50 µL Whole blood.
- Substrate / isotope
- N/A
| instructions for use |
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Intended Use
This Immunosuppressants kit is intended for the determination of four Immunosuppressant medications in whole blood, conducted by laboratory professionals on LC-MS/MS.
Summary
Immunosuppressant drugs suppress the immune response in humans. The most common immunosuppressants are the calcineurine inhibitors Cyclosporin and Tacrolimus, and the mTOR inhibitors Sirolimus and Everolimus.
Mass spectrometry based methods have been tested for the determination of Immunosuppressants. In this analytical method, 4 immunosuppressants are determined from human whole blood by LC-MS/MS. Prior to the LC-MS/MS analysis, a sample clean-up is performed to remove the sample matrix and to spike the samples with the internal standard. The prepared samples are injected into the UHPLC system and, after separation by chromatography on an analytical C18 column, the compounds are ionized by electrospray ionization (ESI) and detected by MS/MS.
An isotopic version of the measurand, dissolved in a deproteinization solution. Used to identify and correct potential deviating values, due to errors or varying circumstances in sample preparation or within the LC-MS/MS. The deproteinization solution furthermore deproteinizes the sample to prevent pollution in the LC-MS/MS.
Kit Composition
Two different concentrated solvent kits, as well as single components, are available to order.
Starter Kit
This Kit includes everything necessary for a first installation of the Immunosuppressants LC-MS/MS kit on an LC-MS/MS system. Includes all solvents for HPLC and sample extraction, Calibrators, Controls, as well as one HPLC column and consumables.
Solvent Kit
The Solvent Kit includes a set of solvents to perform the sample extraction and the HPLC. Calibrators, Controls, Consumables, or an HPLC-Column are not included.
Furthermore, all components can be ordered separately.*
Kit Components Table
For concrete data please consult the Instruction for Use in the download box on the top right side.
Our Product Families
Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
And as experts in laboratory automation, we can support our customers with the protocols for open ELISA platforms, such as the Freedom EVOlyzer or Thunderbolt®.
All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).
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As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
Our range of high-quality immunoassays is supported by a diverse portfolio of automated solutions, making us the perfect partner for you and your customers.
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