Steroid Panel LC-MS
- Regulatory Status
- RUO
- Kit size
- 96
- Method
- LC-MS
- Incubation time
- LC-MS run time 10 min + 6 min
- Standard range
- see below
- Specimen / Volumes
- 250 µL serum or plasma (EDTA)
- Substrate / isotope
- NA
One single kit to quickly and reliably determine 18 different steroids with only one extraction process!
In our two videos, each about 4:40 long, we introduce to you this new product line and explain the workflow.
The Steroid Panel LC-MS Kit is a reagent kit for the measurement of 18 steroids from human plasma (EDTA) and serum using solid phase extraction and liquid chromatography-mass spectrometry technology. The analytes are 17 naturally occurring steroid hormones including 11-deoxycorticosterone 11-deoxycortisol, 17-hydroxypregnenolone, 17-hydroxyprogesterone, 21-deoxycortisol, aldosterone, androstenedione, corticosterone, cortisol, cortisone, dehydroepiandrosterone, dehydroepiandrosterone sulfate, dihydrotestosterone, estradiol, estrone, progesterone, testosterone and the synthetic glucocorticoid drug dexamethasone.
Steroid hormones are important signaling molecules that regulate many important physiological and the underlying biochemical processes. They are typically divided into two large groups based on their primary physiological roles: corticosteroids mostly from adrenal cortex and sex steroids mostly from gonads and placenta. Corticosteroids are further divided into mineralocorticoids and glucocorticoids. The former is involved in salt and water balance, while the latter is involved in energy metabolism. Sex steroids are further categorized into progestogens, androgens and estrogens for their roles in pregnancy, male and female sexual developments. Measurement of steroids as physiological markers for fertility is helpful for monitoring health status. Steroid hormones exert their functions by binding to their respective receptors and trigger either gene expression events or kinase signaling
The enzymes responsible for the biosynthesis of steroid hormones can be classified into two groups, cytochrome P450 (CYP) and hydroxysteroid dehydrogenases (HSD).
LC-MS based assays have evolved into the gold standard and considered state of the art in steroid hormone testing.
The calibrator ranges covered by this kit are:
| Analyte | Calibrator ranges ng/mL |
|---|---|
| 11-Deoxycorticosterone | 0.04 - 5.13 |
| 17-hydroxyprogesterone | 0.10 - 12.82 |
| 21-Deoxycortisol | 0.10 - 12.82 |
| Aldosterone | 0.10 - 4.59 |
| Androstenedione | 0.10 - 12.82 |
| Corticosterone | 0.30 - 38.47 |
| Dexamethasone | 0.50 - 64.12 |
| DHEA | 1.0 - 45.9 |
| DHEAS | 50 - 6412 |
| Dihydrotestosterone | 0.15 - 1.57 |
| Cortisone | 0.5 - 64.1 |
| Cortisol | 2.0 - 256.5 |
| Progesterone | 0.10 - 12.82 |
| 11-Deoxycortisol | 0.10 - 12.82 |
| Testosterone | 0.04 - 13.42 |
| 17-Hydroxypregnenolone | 0.30 - 38.47 |
| Estrone | 0.01 - 1.28 |
| Estradiol | 0.03 - 3.85 |
For research use only. Not for use in diagnostic procedures.
For concrete data please consult the Instruction for Use in the download box on the top right side.
Our Product Families
Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
And as experts in laboratory automation, we can support our customers with the protocols for open ELISA platforms, such as the Freedom EVOlyzer or Thunderbolt®.
All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).
This is who we are.
As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
Our range of high-quality immunoassays is supported by a diverse portfolio of automated solutions, making us the perfect partner for you and your customers.
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At Tecan, we are driven to improve people’s lives and health.