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Summary
α-Klotho, named after the Greek Goddess of Fate, Clotho, who spins the thread of life, was originally characterized as an aging suppressor gene. Our ELISA is perfectly suited for the measurement of the product of this gene - the α-Klotho protein. α-Klotho is also known as the messenger of healthy bodies (Fantuzzi G Front Immunol. 2014 Jul 21;5:351).
A defect in klotho gene expression in the mouse results in a syndrome that resembles human ageing, including a short lifespan, infertility, arteriosclerosis, skin atrophy, osteoporosis and emphysema. The absence of α-Klotho leads to a substantially lower bone density than usual (Kuro-o M et al. Nature. 1997 Nov 6;390(6655):45-51).
Since the discovery of α-Klotho, which was described in the publication mentioned above, several research areas have emerged which benefit from the determination of α-Klotho in terms of gaining valuable additional information:
α-Klotho in the light of bone metabolism
α-Klotho and FGF23 are the main mediators of calcium and phosphate homeostasis. Any kind of disturbance in this regard, such as a reduced or increased production of α-Klotho, leads to metabolic bone disorders (John GB et al. Am J Kidney Dis. 2011 Jul;58(1):127-34).
It is already known that α-Klotho levels in blood decrease with age (Yamazaki Y et al. Biochem Biophys Res Commun. 2010 Jul 30;398(3):513-8). Low α-klotho levels are often associated with age-related diseases.
α-Klotho in kidney context
The outcomes of research in the areas of bone metabolism and aging, as well as the fact that α-Klotho is produced exclusively in the kidneys and brain, have prompted investigations into the impact of changes in α-Klotho levels in renal disease. Studies have revealed that α-klotho levels are significantly decreased in the serum already at an early stage in chronic kidney disease (Pavik I et al. Nephrol Dial Transplant. 2013 Feb;28(2):352-9). Since chronic kidney disease frequently occurs secondary to other medical conditions, α-Klotho is receiving increasing attention as a marker of the occurrence of chronic kidney disease in primary diseases such as type 2 diabetes (Lee EY et al PLoS One. 2014 Aug 1;9(8):e102984), whereby the sample material should be noted (see image below).
α-Klotho in the context of longevity
Several studies have shown that elevated α-Klotho levels can be linked to improved cognitive and brain resilience. People with higher levels have been shown to be less prone to any type of brain damage, including infections and neurodegenerative diseases.
Recent research from the FIT-AGEING study in Spain shows that engaging in moderate physical activity is the most effective way to boost alpha-Klotho. In contrast, both a sedentary lifestyle and excessive exercise fail to deliver the same benefits. This insight helps to make informed choices that truly impact long-term health.
α-Klotho is a biomarker of interest in research related to aging biology and cellular processes. Widely used in aging and longevity research, the IBL-Japan α-Klotho ELISA is a well-established assay, cited in over 400 publications for soluble α-Klotho measurement.
Separate control set available on request
Distributed by TECAN, IBL International
For concrete data please consult the Instruction for Use in the download box on the top right side.
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