17ß-Estradiol RIA (CT)
- Regulatory Status
- EU: CE
- Kit size
- 96
- Method
- RIA (CT)
- Incubation time
- 1 x 3 h
- Standard range
- 9 - 3900 pg/mL
- Specimen / Volumes
- 50 µL serum
- Substrate / isotope
- 125I < 142 kBq
Intended use
Radioimmunoassay for the quantitative measurement of human Estradiol (E2) in serum.
Biological Activity
17-beta-estradiol (E2) is a C-18 steroid hormone (molecular weight 272.4 Da) produced mainly by the ovary and placenta, and in small amounts by adrenals and testes. Estradiol is in equilibrium with estrone, which can be converted to estriol by the liver and placenta.
Clinical applications
Like for LH-FSH-progesterone, measurement of estradiol concentration in serum, peritoneal fluid and follicular fluid is an essential biochemical tool for the investigation of fertility, tumor and sexual diseases, and disorders of hypothalamic/pituitary/gonadal axis, for example:- To detect the follicular phase;
- To check the effectiveness of the induction of ovulation (with ultrasound) and the level of E2 in follicular fluid makes it possible to detect normal or dysfunctional ovulation induction (the empty follicle syndrome may reflect a dysfunctional ovulation induction);
- To diagnose the luteinized unruptured follicle (LUF) syndrome (by the estimation of 17 beta-estradiol and progesterone levels in peritoneal fluid)
- To aid in the diagnosis of breast tumors (total estrogens - E1-E2 - and 17 beta-hydroxysteroid dehydrogenase activity are significantly higher in malignant than in non malignant breast tissues);With LH-FSH and E2 levels, it is possible to suspect a Stein Cohen-Leventhal syndrome;
- Other areas of investigation are: premature adrenarche, gynecomastie and menopausal period.
For concrete data please consult the Instruction for Use in the download box on the top right side.
Our Product Families
Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
And as experts in laboratory automation, we can support our customers with the protocols for open ELISA platforms, such as the Freedom EVOlyzer or Thunderbolt®.
All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).
This is who we are.
As part of the Tecan Group, we have a leading market position in diagnostics and research, with over 40 years of experience in the development, manufacture and supply of enzyme-, radiolabel- and luminescence-based immunoassays.
Our range of high-quality immunoassays is supported by a diverse portfolio of automated solutions, making us the perfect partner for you and your customers.
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