Murine TNF-alpha ELISA
- Regulatory Status
- RUO
- Incubation time
- 1 x 3h, 1 x 30min., 1 x 20min.
- Kit size
- 12 x 8
- Specimen / Volumes
- 100 μL supernatants, buffered solutions, serum, plasma
- Standard range
- 31.25 - 1000 pg/mL
- Substrate / isotope
- TMB 450 nm
Intended use
The Murine TNFα ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of murine TNFα (mTNFα) in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant mTNFα.
Summary
A capture Antibody highly specific for mTNFα has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of mTNFα samples and known standards to the capture antibodies and subsequent binding of the Biotinylated anti-mTNFα secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed.
The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing.
A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of mTNFα present in the samples and standards.
The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of mTNFα in any sample tested.
This kit has been configured for research use only. Not suitable for use in therapeutic procedures.
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Our comprehensive immunoassay portfolio includes a number of specialty diagnostic immunoassays for endocrinology, immunology and autoimmunity, as well as for diagnosis of multiple infectious diseases. We are pioneers and market leaders in saliva diagnostics, with over 40 years of experience supplying a broad portfolio of luminescence- and ELISA-based tests, including our highly acclaimed HMGB1 and MuSK-Ab ELISAs.
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All products are only available for sale to laboratory professionals and may not be available in all countries. Availability and regulatory status may vary across regions depending on local country-specific registration. Please always read and follow the instructions for use.
All of our assays have been designed and manufactured to meet the highest global regulatory requirements and quality standards. Tecan is certified under ISO 9001:2015, ISO 13485:2016 and is audited by a notified body according to Medical Device Single Audit Program (MDSAP).
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