Free 25-OH Vitamin D ELISA
- Regulatory Status
- EU: CE
- Kit size
- 12 x 8
- Method
- ELISA
- Incubation time
- 1 x 90 min, 1 x 30 min, 1 x 20 min
- Standard range
- 4 -42.5 pg/mL
- Specimen / Volumes
- 10 µL serum
- Substrate / isotope
- TMB 450 nm
Get rid of inaccurate calculations - The only direct measurement of free 25-OH Vitamin D by ELISA
Summary
About 90% of the total circulating 25-OH Vitamin D is bound to the so-called VDBP or DBP. The remaining 10% are bound to albumin, the main protein of human blood plasma.
A tiny fraction representing 0.04% of the total 25-OH Vitamin D concentration circulates as a free form. This fraction of free 25-OH Vitamin D relates to the biological activity of Vitamin D, and therefore may better reflect the physiological action of Vitamin D than the total concentration of 25-OH Vitamin D.
Intended use
The Free 25-OH Vitamin D ELISA is a quantitative immunoassay for in vitro determination of the concentration of free 25-OH Vitamin D in human Serum.
Simple, accurate and direct
Why you should use the Free 25-OH Vitamin D ELISA. Following methods for measuring free 25-OH Vitamin D exist:
- Centrifugal ultrafiltration – accurate but long and tedious
- Calculations – requires three assays and can be inaccurate
- Direct measurement by ELISA – simple and accurate
The Free 25-OH Vitamin D Elisa is based on the use of proprietary anti-25-OH D2/D3 monoclonal antibody and offers a direct method for measuring free 25-OH Vitamin D in human serum samples. Clinical studies have been indicating that the measurement of free 25-OH Vitamin D in serum samples of e.g.,
- pregnant women
- haemodialysis patients
- CDK-patients and
- patients with liver failure
provides more relevant diagnostic information than measurement of routine Total 25-OH Vitamin D assays.
Distributed by TECAN, IBL International
For concrete data please consult the Instruction for Use in the download box on the top right side.
- McWhorter, C. A., Mead, M. J., Rodgers, M. D., Ebeling, M. D., Shary, J. R., Gregoski, M. J., Newton, D. A., Baatz, J. E., Hollis, B. W., Hewison, M., & Wagner, C. L. (2023).
Predicting comorbidities of pregnancy: A comparison between total and free 25(OH)D and their associations with parathyroid hormone.
The Journal of steroid biochemistry and molecular biology, 235, 106420.
DOI: 10.1016/j.jsbmb.2023.106420
PubMed ID: 37913892 - Sempos, C. T., Lindhout, E., Heureux, N., Hars, M., Parkington, D. A., Dennison, E., Durazo-Arvizu, R., Jones, K. S., & Wise, S. A. (2022).
Towards harmonization of directly measured free 25-hydroxyvitamin D using an enzyme-linked immunosorbent assay.
Analytical and bioanalytical chemistry, 414(27), 7793–7803.
DOI: 10.1007/s00216-022-04313-y
PubMed ID: 36109397 - Tian, M., Zeng, S., Cai, S., Reichetzeder, C., Zhang, X., Yin, C., Kuang, W., Cheng, K., Jiang, Y., Tao, M., Zeng, Y., Lin, G., Li, J., Gong, F., & Hocher, B. (2022).
25(OH)VitD and human endocrine and functional fertility parameters in women undergoing IVF/ICSI.
Frontiers in endocrinology, 13, 986848.
DOI: 10.3389/fendo.2022.986848
PubMed ID: 36105399 - Jonsdottir, G. M., Kvaran, R. B., Skarphedinsdottir, S. J., Karason, S., Krueger, D., Coursin, D. B., Binkley, N., Hoofnagle, A. N., Hogan, K., Sigurdsson, G. H., & Sigurdsson, M. I. (2022).
Changes in vitamin D metabolites at the time of critical illness and 6 months later-A prospective observational study.
Acta anaesthesiologica Scandinavica, 66(10), 1202–1210.
DOI: 10.1111/aas.14137
PubMed ID: 36054671
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