Longevity Biomarkers
for Healthy Aging.

DHEA (dehydroepiandrosterone) and its sulphate ester DHEA-S serve as key precursors for testosterone and estrogens. Levels rise during adrenarche, peak in early adulthood and then decline steadily with age, reaching extremely low levels by age 80. This marked age-related decrease is why DHEA and DHEA-S are often called "anti-aging hormones".

DHEA and cortisol balance.

DHEA demonstrates immunomodulatory, antidiabetic, neuroprotective and potential anti-aging effects.² Aging triggers a hormonal shift — DHEA-S decreases while cortisol rises — affecting brain, bone and cardiovascular health.³ Studies, including Heaney et al., 2012,⁴ show that decreased DHEA and a higher cortisol/DHEA ratio are linked to cognitive and immune impairments, reduced daily functioning and increased infection risk in older adults under chronic stress.

Steroid hormone assessment from saliva allows the specific determination of the biologically active ("free") fraction of target hormones. Non-invasive saliva sampling enables convenient longitudinal monitoring and supports patient compliance in preventive health programs.

Highly sensitive and validated saliva immunoassays, with clearly defined normal values, enable reliable and convenient assessment of biologically active steroid hormones.

Measurement of DHEA and cortisol provides quantitative data on adrenal hormone levels, supporting the assessment of adrenal function and hormone balance. DHEA measurement also aids in evaluating hormone imbalances and physiological status in adults.

Titin is the largest protein (3,800 kDa) in the human body and is found in muscle cells. It acts like a spring, helping muscles stretch and then return to their normal shape. Titin plays a key role in making muscles strong and flexible.

Titin in the context of longevity.

Loss of muscle mass and function (sarcopenia) is one of the major drivers of frailty and loss of independence during aging. Titin N-Fragment is an emerging biomarker of muscle degradation that enables early assessment and monitoring of muscle health.

Sarcopenia is defined as follows:

• Age-related disease involving progressive loss of skeletal muscle mass, strength and physical function.
• Diagnosable and treatable condition, with muscle mass index as a key measurement tool.
• It affects approximately 16–29% of individuals over 80 years of age.

Titin N-Fragment can be measured in urine or serum as a biomarker of interest in research related to muscle protein turnover and skeletal muscle biology.

Sarcopenia, frailty and muscle decline are active areas of scientific research, with studies investigating factors such as nutrition and physical activity. Urine and serum-based assays provide laboratories with tools for the quantitative measurement of Titin N-Fragment.

Sarcopenia and frailty are not inevitable. Evidence highlights the preventive impact of a protein-rich diet, vitamin D and targeted exercise.^{6, 7}

α-Klotho, named after the Greek myth Clotho, who spins the thread of life, is widely recognized as a longevity-associated protein linked to kidney function, vitamin D metabolism and cognitive resilience. Reduced α-Klotho levels have been associated with age-related decline and chronic disease.

Altered α-Klotho levels have been associated with kidney dysfunction,^{8, 9} metabolic disease and age-related decline, highlighting its relevance as a biomarker in longevity research.

Several studies^10–12 have shown that elevated alpha-Klotho levels can be linked to improved cognitive and brain resilience. People with higher levels have been shown to be less prone to any type of brain damage, including infections and neurodegenerative diseases.

Recent research from the FIT-AGEING study^13–15 in Spain shows that engaging in moderate physical activity is the most effective way to boost alpha-Klotho. In contrast, both a sedentary lifestyle and excessive exercise fail to deliver the same benefits.

α-Klotho is a biomarker of interest in research related to aging biology and cellular processes. Widely used in aging and longevity research,^13–15 the IBL-Japan α-Klotho ELISA is a well-established assay, cited in over 400 publications for soluble α-Klotho measurement.

Vitamin D plays an important role in bone health, immune function and overall well-being. Accurate assessment of vitamin D status is therefore an important part of patient evaluation.

Total 25-OH vitamin D measurement may not fully reflect vitamin D bioavailability in all individuals. Measurement of free 25-OH vitamin D provides additional insight into the biologically active fraction and supports a more personalized assessment of vitamin D status.¹⁶

suPAR (soluble urokinase Plasminogen Activator Receptor) is a protein found in blood, reflecting the level of immune system activity. It is the shed membrane-bound receptor uPAR from immune and senescent (aged) cells.

Chronic low-grade inflammation ("inflammaging") is a major driver of biological aging and age-related disease.¹⁷ suPAR is an innovative biomarker reflecting persistent immune activation and systemic inflammation.

Elevated suPAR signals faster aging, increased disease risk and higher mortality, while low levels reflect robust health and slower aging. suPAR's predictive power surpasses traditional markers, offering actionable insights — levels can be lowered through lifestyle changes such as exercise, diet, stress management and smoking cessation.^{18, 19}

The soluble Interleukin-2-Receptor ELISA is intended for the quantitative determination of human soluble IL-2 receptor (sIL-2R) in serum and plasma (EDTA, citrate, heparin). sIL-2R levels can be used as a physiological marker for the indication of inflammation in the body, in-vivo immune system activation and response activity.

The interleukin-2 receptor (IL-2R) is a monomeric, heterodimeric or trimeric protein expressed on the surface of certain immune cells like lymphocytes that binds and responds to a cytokine called IL-2. Interleukin-2 (IL-2) is a body-own signaling molecule mainly produced by activated T-cells.